Cultural monuments are one of the strongest stakeholders at the regional level of tourism in the Czech Republic. There is a huge potential for cooperation between destination management and cultural monuments at the regional level. However, these possibilities are not fully established yet. The article is based on the survey conducted among managers of cultural monuments in the selected region of the Czech Republic to demonstrate the importance of cooperation between these two subjects. On the basis of the survey and analysis of the possibilities of cooperation between tourism industry and cultural monuments at the regional level, the new ways of cooperation are recommended.