In their paper the authors focused on job satisfaction with regard to the implementation of motivational and hygiene factors towards subordinate managers, as developed by Herzberg in his two-factor theory. The authors conducted an inquiry to find out whether particular motivational factors, as well as hygiene factors, evoke in managers a feeling of satisfaction, dissatisfaction or a neutral attitude. Furthermore, the authors focused on ascertaining whether there is a correlation between the assessments of satisfaction within particular factors and the gender, age, educational level, duration of management practice, level of management, position in a separate hotel or a hotel chain, a hotel category, and size of the hotel. The data were arranged into groups in order to emphasize the characteristic features of examined phenomena. Statistical methods to examine the correlations were applied to verify or disprove the given hypotheses. The research outcomes confirmed different impacts of motivational and hygiene factors, even in respondents who hold managerial positions in hotels and hotel chains. One or two statistical correlations dealing with some above mentioned criteria were found in ten out of fourteen examined factors. When dividing respondents into groups according to the level of management, a statistical correlation of four factors was found, and a further three relationships were identified while dividing the respondents into groups by their age and hotel category. A statistical correlation was not proved between motivational and hygiene factors and level of education.