The article deals with the evaluation of the extent of regional development support from public funds during the programming period EU 2007-2013, and in culture, specifically into its parts, and in galleries, museums, memorials and monuments. Altogether in this area 12.2 billion CZK was invested, with 72% of the subsidies from public funds. Most of the public funds were invested in cultural offerings in the Central Region, the least in the Karlovy Vary region. The highest ability to elicit additional funds through public sources has been demonstrated in South Moravia. The framework of efficiency of expenditure of public funds can be evaluated on the basis of index analysis, which showed that on average 576 thousand CZK per visitor was invested and nearly 146 thousand CZK per employee from public sources.